ارزش تشخیصی تست gyrB-RFLP PCR در تعیین گونه مایکوباکتریوم‌های بیماریزا در بیماران مسلول در استان مازندران

Background and purpose: Mycobacterium tuberculosis complex (MTBC) members are causative agents of human and animal tuberculosis. Differentiation of MTBC members is essential for appropriate treatment of individual patients and reduce drug resistance. Materials and methods: A total of 1345 samples were collected from patients clinically suspected of contracting tuberculosis that referred to health care center of Mazandaran from July 2010 to June 2011. The specimens were stained by the Ziehl-Neelsen staining technique and were cultured on Lowenstein-Jensen medium to detect the mycobacteria. For recognition of Mycobacterium tuberculosis complex members, MTUB-f and MTUB-r primers (gyrB-PCR1) were used. For differentiation of Mycobacterium tuberculosis complex members, MTUB-756-Gf and MTUB- 1450Cr (gyrB-PCR2) and RFLP PCR using RsaI restriction enzyme were used. Results: Of 1345 specimens, only 65(4.83%) isolates were positive culture. Out of 65, 59 (90.76%) were MTBC and 6 (9.24%) identified as Mycobacteria other than tuberculosis. All of 59 isolates were M. tuberculosis and showed the typical RsaI pattern. Conclusion: The gyrB-RFLP PCR and the RsaI restriction enzyme is a rapid and easy technique for differentiation of the members of M. tuberculosis complex which is crucial for rapid treatment of patients.